Three-dimensional cell culture technique and pathophysiology
نویسندگان
چکیده
منابع مشابه
three-dimensional (3d) cell culture conditions, present and future improvements
conclusions our underlying hypothesis is that recapitulating the three-dimensional early embryonic structure, in order to reproduce better in vitro the three-dimensional morphogenetic-like re-arrangements, would improve cells differentiation, when in vivo transplanted; moreover, it could be used as a simplified cancer disease model and reliable drug evaluation method as well. results the proces...
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Techniques that allow cells to self-assemble into three-dimensional (3-D) spheroid microtissues provide powerful in vitro models that are becoming increasingly popular--especially in fields such as stem cell research, tissue engineering, and cancer biology. Unfortunately, caveats involving scale, expense, geometry, and practicality have hindered the widespread adoption of these techniques. We p...
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Cells, grown as monolayers (2D models), are routinely used as initial model systems for evaluating the effectiveness and safety of libraries of molecules with potential as therapeutic drugs. While this initial screening precedes preclinical animal studies before advancing to human clinical trials, cultured cells frequently determine the initial, yet crucial, 'stop/go' decisions on the progressi...
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ژورنال
عنوان ژورنال: Advanced Drug Delivery Reviews
سال: 2014
ISSN: 0169-409X
DOI: 10.1016/j.addr.2014.01.003